During this week we will review this topics and execute hands on lab work
Laboratory Safety & Good Laboratory Practices (GLP)
- Basic lab safety rules and PPE usage
- Sterile techniques and contamination prevention
- Waste disposal and biosafety levels
Introduction to Essential Laboratory Equipment
- Pipetting techniques (micropipettes, serological pipettes)
- Centrifuges and ultracentrifugation principles
- Spectrophotometers and nanodrop usage for nucleic acid quantification
- pH meters and buffer preparation
Molecular Biology Techniques for mRNA Work
- Nucleic acid extraction (RNA/DNA purification techniques)
- Gel electrophoresis for RNA/DNA analysis
- Polymerase Chain Reaction (PCR) and quantitative PCR (qPCR) basics
- Reverse transcription for mRNA synthesis
Cell Culture & Transfection Methods
- Aseptic techniques and handling of mammalian cell culture
- Media preparation and cell viability assays
- Transfection techniques (lipid nanoparticles, electroporation)
- Cryopreservation and thawing of cells
Protein and Nucleic Acid Analysis
- Western blotting for protein detection
- ELISA for antigen-antibody interactions
- High-Performance Liquid Chromatography (HPLC) for mRNA purity analysis
- Mass spectrometry basics for protein identification
Introduction to Bioprocessing & mRNA Production
- In vitro transcription (IVT) for mRNA synthesis
- mRNA purification methods
- Lipid nanoparticle (LNP) formulation for mRNA delivery
- Quality control in mRNA production