This week, we will review the following subjects
DNA Cloning Techniques
- Restriction enzyme digestion and ligation.
- Transformation methods: Chemical and electroporation.
- Selection markers and screening methods.
Cell Line Development
- Establishment of bacterial and mammalian cell lines.
- Transfection and selection of stable cell lines.
- Cell line characterization and authentication.
GMP Considerations
- Importance of cell line stability and consistency.
- Documentation and traceability requirements.
- Regulatory guidelines for cell line development
Laboratory Work: Real-World Application
- Example: Development of CHO cell lines for biopharmaceutical production