This week we will be going over the following subjects:
Plasmid DNA Amplification:
- Large-scale bacterial culture conditions.
- Induction of plasmid replication.
- Harvesting and lysis methods.
Plasmid Purification:
- Alkaline lysis and purification protocols.
- Chromatographic techniques: Ion exchange and affinity chromatography.
- Quality assessment: Purity, endotoxin levels, and integrity.
GMP Considerations:
- Validation of purification processes.
- In-process controls and documentation.
- Compliance with regulatory standards.
Laboratory Work Real-World Application:
- Case study: Plasmid production for gene therapy applications.