This week we will be going over the following subjects:
LNP Design and Composition:
- Lipid selection: Ionizable lipids, cholesterol, and PEGylated lipids.
- Formulation methods: Microfluidic mixing and ethanol injection.
- Optimization of lipid ratios for effective delivery.
Characterization of LNPs:
- Size and zeta potential measurement.
- Encapsulation efficiency and loading capacity.
- Stability studies under various storage conditions.
GMP Considerations:
- Scale-up challenges and process validation.
- Documentation of formulation parameters.
- Regulatory requirements for LNP-based delivery systems.
Laboratory Work Real-World Application:
- Case study: LNP formulation for mRNA COVID-19 vaccines.