This week we will be going over the following subjects:
Complexation Process:
- Mixing conditions: Time, temperature, and lipid-to-mRNA ratio.
- Monitoring encapsulation efficiency.
- Optimization for maximum delivery efficacy.
Purification of mRNA-LNPs:
- Removal of unencapsulated mRNA and free lipids.
- Chromatographic and filtration techniques.
- Quality control: Particle size distribution and endotoxin testing.
GMP Considerations:
- In-process monitoring and control.
- Documentation of purification steps.
- Compliance with sterility and endotoxin limits.
Laboratory Work Real-World Application:
- Example: Purification strategies for mRNA vaccines.